Because oglio manufacturing is an emerging field, it comes with a number of development and application challenges:
- Delivery of RNAs across the lipid bilayer and into cells
- High rates of degradation of oligos by plasma and tissues
Drug delivery into the target cells requires the proper osmotic pressure, similar to the parameters around viral transfection in vector development. The optimal osmolality of the delivery complex (e.g. mRNA:PEI) should be determined and monitored during this process. This specification will provide repeatable successful delivery of the drug.